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Meteorological, albedo and evapotranspiration data set of hulugou shrub experimental area in the upper reaches of Heihe River (2012-2014)

The data set is the meteorological and observational data of hulugou shrub experimental area in the upper reaches of Heihe River, including meteorological data, albedo data and evapotranspiration data under shrubs. 1. Meteorological data: Qilian station longitude: 99 ° 52 ′ E; latitude: 38 ° 15 ′ n; altitude: 3232.3m, scale meteorological data from January 1, 2012 to December 31, 2013. Observation items include: temperature, humidity, vapor pressure, net radiation, four component radiation, etc. The data are daily scale data, and the calculation period is 0:00-24:00 2. Albedo: daily surface albedo data from January 1, 2012 to July 3, 2014, including snow and non snow periods. The measuring instrument is the radiation instrument on the 10m gradient tower in hulugou watershed. Among them, the data from August 4 to October 2, 2012 was missing due to instrument circuit problems, and the rest data quality was good 3. Evapotranspiration: surface evapotranspiration data of Four Typical Shrub Communities in hulugou watershed. The observation period is from July 18 to August 5, 2014, which is the daily scale data. The data include precipitation data, evaporation and infiltration data observed by lysimeter. The data set can be used to analyze the evapotranspiration data of alpine shrubs and forests. The evapotranspiration of grassland under canopy was measured by a small lysimeter with a diameter of 25 cm and a depth of 30 cm. Two lysimeters were set up in each shrub plot, and one lysimeter was set for each shrub in transplanting experiment. The undisturbed undisturbed soil column with the same height as the barrel is placed in the inner bucket, and the outer bucket is buried in the soil. During the embedding, the outer bucket shall be 0.5-1.0 cm higher than the ground, and the outer edge of the inner barrel shall be designed with a rainproof board about 2.0 cm wide to prevent surface runoff from entering the lysimeter. Lysimeter was set up in the nearby meteorological stations to measure grassland evapotranspiration, and a small lysimeter with an inner diameter of 25 cm and a depth of 30 cm was also set up in the sample plot of Picea crassifolia forest to measure the evaporation under the forest. All lysimeters are weighed at 20:00 every day (the electronic balance has a sensing capacity of 1.0 g, which is equivalent to 0.013 mm evaporation). Wind proof treatment should be taken to ensure the accuracy of measurement. Data processing method: evapotranspiration is mainly calculated by mass conservation in lysimeter method. According to the design principle of lysimeter lysimeter, evapotranspiration is mainly determined by the quality difference in two consecutive days. Since it is weighed every day, it is calculated by water balance.

2020-07-31

Moisture absorption and utilization dataset of desert plants in Heihe River Basin (2014-2015)

The data set of atmospheric water vapor absorption and utilization of desert plants, all of which are original data, including the liquid flow and environmental data of wild desert plants (Sitan village and Ejina Banner, Jingtai County), such as Tamarix, Bawang, Baici, Hongsha, etc., including the data of meteorology, photosynthesis, fluorescence and leaf surface humidity, as well as the data of gene transcriptome and expression regulation.

2020-07-31

Field LAI dataset in the Heihe River basin (2011)

The dataset is Lai data of ground sample points in Heihe River Basin, collected by LAI-2000 canopy analyzer. The collection area is located in Zhangye rural demonstration base, Ejina Banner, Jiuquan Satellite Center (2011) and other areas. The main measured vegetation is corn. The Lai value of maize was obtained by using lai2000, and the observation was repeated twice in the mode of one up four down. Cd202 was used to obtain the leaf area of each leaf of maize plant, and three maize plants were collected.

2020-07-30

Dataset of shrub interception and transpiration in Tianlaochi watershed of Qilian Mountain (2012)

This data includes three parts of data, namely shrub water holding experiment, shrub interception experiment and shrub transpiration experiment data. Shrub water holding experiment: select the two shrub types of Caragana jubata and Potentilla fruticosa, respectively pick the branches and leaves of the two vegetation types, weigh their fresh weight, carry out water holding experiment, measure the saturated weight of branches and leaves, dry weight of branches and leaves, dry weight of branches and leaves after completion, and finally obtain the data of branches, leaves and total water holding capacity. Shrub interception experiment: two shrubs, Caragana jubata and Potentilla fruticosa, were also selected and investigated. 30 rain-bearing cups were respectively arranged under the two shrubs. after each rainfall, penetration rainfall was measured and observed from June 1, 2012 to September 10, 2012. Shrub Transpiration Experiment: Potentilla fruticosa on July 14, Caragana jubata on August 5, Salix gilashanica on August 15, 2012. The measurement is made every hour according to the daily weather conditions.

2020-07-30

Water vapor absorption and utilization data set of desert plants in Heihe River Basin (2012-2014)

All data in this data set are original data, including meteorological and soil moisture content, stem sap flow, water potential of plant tissue, isotope characteristics of atmospheric and humidified water vapor, fluorescence tracer image, plant photosynthetic fluorescence, and basic data of five desert plants, Tamarix chinensis, Haloxylon ammodendron, Bawang, Nitraria tangutorum and red sand, which are related to field and indoor control experiments Because of the data of expression regulation. 1. Isotopic data of Tamarix chinensis. After humidifying for 1 hour, 2 hours and 3 hours, the tissue samples of indoor and outdoor plants of plexiglass were collected at the same time. The samples were put forward and processed by low-temperature vacuum distillation glass water extraction system, and then used euro The isotopic data were measured by ea3000 element analyzer and isoprime gas stability mass spectrometer. Tamarix Tamarix samples were collected from Sitan village, Jingtai County, including humidification and control samples. The variation data of isotopic composition can be used to determine the way and amount of water vapor absorbed by plant leaves. 2. Fluorescence section photo data: all the data in this data set are original data, including the structural photos under high-power microscope of Tamarix, Haloxylon ammodendron, Nitraria, Bawang, Hongsha and other desert plant leaves in Sitan village of Jingtai County and Ejin Banner. The specific method is as follows: apply fluorescent dye to the surface of desert plant leaves before humidification, collect plant leaves and stems after humidification for 1 hour, 2 hours and 3 hours, put them in liquid nitrogen, take them back to the laboratory, observe and take photos with fluorescence microscope. It can be used to analyze the tissue and organs of water absorption by desert plant leaves and the direction and path of water migration in plants. 3: Gene transcription and expression data: transcription and expression data of Tamarix chinensis, data collection time: May 25, 2014, location: Sitan village, Jingtai County, Gansu Province, data analysis platform: lllumina hisep TM 2000 platform, obtained by transcriptome analysis of baimaike company. 4. Photosynthetic and fluorescence data: photosynthetic and fluorescence parameters measured by photosynthetic apparatus in the field (Sitan village and Ejin Banner, Jingtai County). 5. Sap flow and environmental data: all data are original data. Sap flow data of desert plants measured by stem flow meter, including Tamarix chinensis, Haloxylon ammodendron, Nitraria tangutorum, red sand and other desert plants (Sitan village, Jingtai County and Ejin Banner), and environmental data monitored by automatic weather station, including temperature and humidity.

2020-07-30

Soil observation and leaf area index and aboveground biomass of maize sampling points in Yingke Daman area of Heihe River Basin (2012)

The experimental data of Yingke Daman in Heihe River Basin is supported by the key fund project of Heihe River plan, "eco hydrological effect of agricultural water saving in Heihe River Basin and multi-scale water use efficiency evaluation". Including: soil bulk density, soil water content, soil texture, corn sample biomass, cross-section flow, etc Data Description: 1. Sampling location of Lai and aboveground biomass: Yingke irrigation district; sampling time: May 2012 to September 2012; Lai and aboveground biomass of maize were measured by canopy analyzer (lp-80), and aboveground biomass was measured by sampling drying method; sample number: 16. 2. Soil texture: Sampling location: Yingke irrigation district and Shiqiao Wudou Er Nongqu farmland in Yingke irrigation district; soil sampling depth is 140 cm, sampling levels are 0-20 cm every 10 cm, 20-80 cm every 20 cm, 80-140 cm every 30 cm; sampling time: 2012; measurement method: laboratory laser particle size analyzer; sample number: 38. 3. Soil bulk density: Sampling location: Yingke irrigation district and Daman irrigation district; sampling depth of soil bulk density is 100 cm, sampling levels are 0-50 cm and 50-100 cm respectively; sampling time: 2012; measurement method: ring knife method; number of sample points: 34. 4. Soil moisture content: this data is part of the monitoring content of hydrological elements in Yingke irrigation district. The specific sampling location is: Shiqiao Wudou Er Nongqu farmland in Yingke Irrigation District, planting corn for seed production; soil moisture sampling depth is 140 cm, sampling levels are 0-20 cm every 10 cm, 20-80 cm every 20 cm, 80-140 cm every 30 cm Methods: soil drying method and TDR measurement; sample number: 17. 5. Cross section flow: Sampling location: the farmland of Wudou Er Nong canal in Shiqiao, Yingke irrigation district; measure the flow velocity, water level and water temperature of different canal system sections during each irrigation, record the time and calculated flow, monitor once every 3 hours until the end of irrigation; sampling time: 2012.5-2012.9; measurement method: Doppler ultrasonic flow velocity meter (hoh-l-01, Measurement times: Yingke irrigation data of four times.

2020-07-30

Cross-section characteristics of plant leaf in the arid areas of middle-lower reaches of Heihe River

The leaf cross-sectional structure of constructive species in arid area of the middle and lower reaches of Heihe River Basin. The material number is consistent with the sampling table. Refer to the sampling table number to determine the material and its distribution position. A semi thin section of 65 dominant plants. The mesophyll structure of C3 and C4 plants, the characteristics of palisade tissue and sponge tissue, as well as the special structure including crystalloid cells can be reflected.

2020-07-30

1:100000 vegetation map of Heihe River Basin(2015) (version 3.0)

1:100000 vegetation map of Heihe River Basin, the regional scope is subject to the Heihe river boundary of Huangwei Committee, the area is about 14.29 × 104km2, the data format is GIS vector format, this version is version 3.0. The data is mainly based on ground observation data, integrated with all kinds of remote sensing data, 1:1 million vegetation map, climate, terrain, landform, soil data mapping, and compiled by cross validation. The classification standard, legend unit and system of vegetation map of the people's Republic of China (1:1000000), 2007 are adopted, including vegetation type group, vegetation type, formation and sub formation. The new version mainly unifies the codes of the new formation (74 codes in total, distinguishing the formation and the sub formation). 9 vegetation type groups, 22 vegetation types and 74 formations (sub formations) in version 2.0 were changed into 9 vegetation type groups, 22 vegetation types and 67 formations (7 sub formations). The data includes versions 2.0 and 3.0

2020-07-30

Data set of plant physiological indexes and soil water, salt and nutrient in the lower reaches of Tarim River (2000-2006)

In the ecosystem, soil and vegetation are two interdependent factors. Plants affect soil and soil restricts vegetation. On the one hand, there are a lot of nutrients such as carbon, nitrogen and phosphorus in the soil. On the other hand, the availability of soil nutrients plays a key role in the growth and development of plants, directly affecting the composition and physiological activity of plant communities, and determining the structure, function and productivity level of ecosystems. Soil moisture content (or soil moisture content): In the 9 sections from Daxihaizi to taitema lake in the lower reaches of Tarim River, plant sample plots are set in the direction perpendicular to the river channel according to the arrangement of groundwater level monitoring wells. Dig one soil profile in each sample plot, collect one soil sample from 0-5 cm, 5-15 cm, 15-30 cm, 30-50 cm, 50-80 cm, 80-120 cm and 120-170cm soil layers from bottom to top in each profile layer, each soil sample is formed by multi-point sampling and mixing of corresponding soil layers, each soil layer uses aluminum boxes to collect soil samples, weighs wet weight on site, and measures soil moisture content (or soil moisture content) by drying method. Soil nutrient: the mixed soil sample is used for determining soil nutrient after removing plant root system, gravel and other impurities, air-drying indoors and sieving. Organic matter is heated by potassium dichromate, total nitrogen is treated by semi-micro-Kjeldahl method, total phosphorus is treated by sulfuric acid-perchloric acid-molybdenum antimony anti-colorimetric method, total potassium is treated by hydrofluoric acid-perchloric acid-flame photometer method, effective nitrogen is treated by alkaline hydrolysis diffusion method, effective phosphorus is treated by sodium bicarbonate leaching-molybdenum antimony anti-colorimetric method, effective potassium is treated by ammonium acetate leaching-flame photometer method, PH and conductivity are measured by acidimeter and conductivity meter respectively (water to soil ratio is 5: 1). Soil water-soluble total salt was determined by in-situ salinity meter. Drought stress is the most common form of plant adversity and is also the main factor affecting plant growth and development. Plant organs will undergo membrane lipid peroxidation under adverse circumstances, thus accumulating malondialdehyde (MDA), the final decomposition product of membrane lipid peroxide. MDA content is an important indicator reflecting the strength of membrane lipid peroxidation and the damage degree of plasma membrane, and is also an important parameter reflecting the damage of water stress to plants. At the same time, under adverse conditions, the increased metabolism of reactive oxygen species in plants will lead to the accumulation of reactive oxygen species or other peroxide radicals, thus damaging cell membranes. Superoxide dismutase (SOD) and peroxidase (POD) in plants can remove excess active oxygen in plants under drought and other adversities, maintain the metabolic balance of active oxygen, protect the structure of the membrane, and finally enhance the resistance of plants to adversities. The analysis samples take Populus euphratica, Tamarix chinensis and Phragmites communis as research objects. According to the location of groundwater monitoring wells, six sample plots are set up starting from the riverside, with an interval of 50 m between each sample plot, which are sample plots 1, 2, 3, 4, 5 and 6 in turn. Fresh leaves of plants are collected, stored at low temperature, and pretreated (dried or frozen) on the same day. PROline (Pro), cell membrane system protective enzymes superoxide dismutase (SOD) and peroxidase (POD) were tested indoors. Preparation of enzyme solution: weigh 0.5g of fresh material and add 4.5mL pH7.8 with ph 7.8. The materials were homogenized in a pre-frozen mortar, which was placed in an ice bath. Centrifuge at 10000 r/min for 15 min. The supernatant was used for determination of superoxide dismutase, peroxidase and malondialdehyde (MDA). PRO determination: put 0.03 g of material into a 20 mL large test tube, add 10mL ammonia-free distilled water, seal it, put it in a boiling water bath for 30min, cool it, filter, filtrate 5 mL+ ninhydrin 5 mL, develop color in boiling water for 60min, and extract with toluene. The extract was colorized with Shimadzu UV-265 UV spectrophotometer at 515 nm. SOD activity was measured by NBT photoreduction. The order of sample addition for enzyme reaction system is: pH 7.8 PBS 2.4mL+ riboflavin 0.2 mL+ methionine 0.2 mL+EDTA0.1 mL+ enzyme solution 0.1 mL+NBT0.2 mL. Then the test tube was reacted under 40001ux light for 20 min, and photochemical reduction was carried out. SOD activity was measured at 650 nm wavelength by UV-265 ultraviolet spectrophotometer. POD activity determination: the reaction mixture was 50 ml PBS with pH 6.0+28 μ L guaiacol+19 UL30% H2O2. 2 mL of reaction mixture +1 mL of enzyme solution, immediately start timing, reading every 1 min, reading at 470 nm. Determination of chlorophyll: ethanol acetone mixed solution method. After cutting the leaves, the mixed solution of 0.2 g and acetone: absolute ethanol = 1: 1 was weighed as the extraction solution. After extracting in the dark for 24 h, the leaves turned white and chlorophyll was dissolved in the extraction solution. The OD value of chlorophyll was measured by spectrophotometer at 652nm. Determination method of soluble sugar: phenol sulfate method is adopted. (1) The standard curve is made by taking 11 20 ml graduated test tubes, numbering them from 0 to 10 points, and adding solution and water according to Table 1 respectively. Then add 1 ml of 9% phenol solution to the test tube in sequence, shake it evenly, then add 5 ml of concentrated sulfuric acid from the front of the tube for 5 ~ 20 s, the total volume of the colorimetric solution is 8 ml, and leave it at constant temperature for 30 minutes for color development. Then, with blank as control, colorimetric determination was carried out at 485 nm wavelength. With sugar as abscissa and optical density as ordinate, a standard curve was drawn and the equation of the standard curve was obtained. (2) Extraction of soluble sugar: fresh plant leaves are taken, surface dirt is wiped clean, cut and mixed evenly, 0.1-0.3 g are weighed, 3 portions are respectively put into 3 calibration test tubes, 5-10 ml distilled water is added, plastic film is sealed, extraction is carried out in boiling water for 3O minutes, the extraction solution is filtered into a 25 ml volumetric flask, repeated flushing is carried out, and the volume is fixed to the calibration. (3) Absorb 0.5 g of sample solution into the test tube, add 1.5 ml of distilled water, and work out the content of soluble sugar in the same way as the standard curve. The amount of solution and water in each test tube Pipe number 0 1-2 3-4 5-6 7-8 9-10 1.100μg/L sugar solution 0.20 0.40 0.60 1.0 2. water/ml 2.0 1.8 1.6 1.4 1.2 1.0 3. Soluble sugar content/μ g 0 20 40 60 80 100 Determination of malondialdehyde: thiobarbituric acid method. Fresh leaves were cut to pieces, 0.5 g was weighed, 5% TCA5 ml was added, and the homogenate obtained after grinding was centrifuged at 3 000 r/rain for 10 rain. Take 2 ml supernatant, add 0.67% TBA 2 ml, mix, boil in 100 water bath for 30 rain, cool and centrifuge again. Using 0.67% TBA solution as blank, the OD values at 450, 532 and 600 nm were determined. Methods for analysis and testing of plant hormones (GA3, ABA, CK, IAA): 0.1 0.005 g plant samples were taken and ground in liquid nitrogen. 500μl methanol was extracted overnight at 4℃. Centrifuge the sample and freeze-dry the supernatant. 30μl10%% CH3CN dissolved the sample. 10μl of sample solution was analyzed by HPLC. The external standard method was used to quantify plant hormones. Standard plant hormones were purchased from sigma Company. See (Ruan Xiao, Wang Qiang, et al., 2000, Journal of Plant Physiology.26 (5), 402-406) for analysis methods.

2020-07-30

Vegetation quadrat survey data in the middle of Heihe River Basin (2013-2014)

The survey data of vegetation quadrat in the middle reaches of Heihe River consists of the field survey data in 2013 and 2014, including the vegetation and soil data of the survey quadrat. The data of each survey sample includes the following information: sample longitude and latitude, sample size, elevation, sample overview, plant name, plant height, crown width, coverage, total coverage, number of trees, plant spacing, row spacing, large row spacing, DBH. The soil is divided into 6 layers according to 0-100cm below the ground, which are 0-10cm, 10-20cm, 20-40cm, 40-60cm, 60-80cm and 80-100cm respectively.

2020-07-30