The birds along elevation gradients in Gangrigabu Mountains were investigated by point count method. With a 400-meter elevational gradient, elevation zones were set up in the survey area. Five elevation zones were built in the north slope from TongMai Town to Galong Temple in Bome County, and 8 elevation zones were built in the south slope from Jiefang Bridge to Galongla in Medog County. So that we can make clear about the pattern and maintenance mechanism of bird diversity along elevation gradients in this region. The data of bird diversity and distribution will be used to further explore the key scientific issues such as the impact of climate change on bird diversity and adaptation strategies, and the response and protection strategies of bird species diversity under the global climate change.
YANG Xiaojun
From October to November 2020, we used both live traps and camera traps to collect mammal diversity and distributions along the elevational gradients at the Yarlung Zangbo Grand Canyon National Nature Reserve. We set trap lines for small mammals inventory, with a total of 8000 live trap nights. We collected 526 individuals and1052 tissue samples of small mammals during the field sampling. We also retrived images of 130 camera traps placed between May 2020 and October 2020. We obtained 4218 pictures of wild animals,25 species of large and medium mammals were recorded.. The camera traps were reset in the same locations after renew batteries and memory cards. Small mammal data consist of richness, abundance, traits, environmental gradients etc, and could be used to model relationship between environmental gradients and traits concatenated by richness matrix. Camera trap data could inventory endangered species in the region, and provide information to identify biodiversity hotspots and conservation priorities.
LI Xueyou
The study of fossils in Bangor and Lunpola is of great significance, and the date of fossils is indispensable. There are volcanic tuffs in this area. Zircon can be used for U-Pb age analysis to determine the age of strata and fossils. This data shows the zircon U-Pb age analysis results of tuff samples from bango and Lunpola fossil sites in a graphical way. The figure shows the shape of a large number of zircons, and indicates the age analysis results on different zircon samples. The data show the large sample size used in related research, and the analysis results are also clear. The image display of this data is intuitive and clear, and the results are reliable, which is of great significance to the study of the Qinghai Tibet Plateau.
SUN Boyang
From April 2020 to August 2020, sub project 3 collected 51 ear tissue samples of Qinghai fine wool sheep distributed in Haiyan County, Haixi Mongolian and Tibetan Autonomous Prefecture, Qinghai Province, 50 blood samples of Oula sheep in Tongde County, Hainan Tibetan Autonomous Prefecture, 50 blood samples of yak in Tongde County, Hainan Tibetan Autonomous Prefecture, 60 blood samples of Haidong donkey in Datong Hui and Tu Autonomous County, Xining City, and tissue samples A total of 211 copies. At the same time, the information of body length, body height, weight, age and gender, as well as the data of economic traits such as litter size, wool fineness and wool length were recorded. The individual photos were taken, and the information of feeding mode and epidemic situation were obtained through questionnaire survey.
TIAN Fei
In order to study the population evolution history and local adaptive genetic mechanism of main domesticated equine animals in Qinghai Tibet Plateau and its surrounding areas, and to establish the corresponding germplasm genetic resource bank. We have sequenced 236 horse samples collected in Qinghai Province, Tibet Autonomous Region and Xinjiang Autonomous Region by the end of 2018, including Tibetan horse, Tibetan donkey, plain domestic donkey and Jiama plain local breed. Seventy five samples (including 73 donkey samples and two horse samples) were sequenced for mitochondrial genome and D-loop sequencing. A number of genomic data were generated by sequencing, which provided data for tracing the domestication, migration, expansion and other historical events of horse domesticated animals in this area, and further exploring the adaptation mechanism of equine animals to the harsh environment such as hypoxia, high temperature and dryness.
LI Yan
In order to describe the distribution pattern of genetic diversity of main domesticated animals in Qinghai Tibet Plateau and its surrounding areas, clarify their genetic background and establish corresponding genetic resource bank. In December 2019, a total of 1208 blood or tissue samples were collected from 254 goats of Chongqing Dazu black goat, Lubei white goat, Hainan black goat, Xuzhou SuBai goat and Yimeng black goat in Chongqing, Shandong, Jiangsu and Hainan, including 9 fresh tissue RNA samples such as heart, liver, spleen, lung and kidney. This data set contains the sample species, species, detailed sampling place, sample type, collection time, collector, storage method and other basic sample information, which is stored in the form of Excel. The data set also contains the appearance photos of individual species, which are stored in JPG format.
PENG Minsheng
In order to describe the distribution pattern of genetic diversity of the main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas (Pan third polar region), and clarify its genetic background. In 2020, we selected 31 RNA tissue samples from kidney, spleen and jejunum of local commercial chicken and native chicken in Kenya. After extracting total RNA, we established a library and performed transcriptome sequencing. Sequencing produced a batch of 180g transcriptome sequencing raw data. In order to explore the domestication, migration, expansion and other historical events of domestic chicken in Pan third pole area, and further explore the adaptation mechanism of domesticated animals to dry and other harsh environment. This data set contains the excel table of basic sample information such as species, breed, sex and phenotype of 31 domestic chicken individuals, and the original data and MD5 value of three tissue transcriptome sequencing of 31 domestic chicken individuals.
PENG Minsheng
In order to describe the distribution pattern of the genetic diversity of the main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas, clarify the related genetic background, and establish the corresponding genetic resource pool. In 2019, 187 samples of blood, tissue and feces of local main domesticated animals were collected in Chayu County, Linzhi Prefecture, Tibet Autonomous Region. This data set contains the basic sample information such as species, varieties, detailed sampling sites, sample types, collection time, collectors and storage methods of domestic chickens, pigs, cattle, horses, dogs and buffaloes in Chayu County, Linzhi, Tibet, and is stored in Excel form. This data set also contains the individual appearance photos of the sample, which are stored in JPG format.
PENG Minsheng YIN Tingting
In order to describe the disease situation of the main domesticated animals in and around the Qinghai Tibet Plateau, the epidemic situation of the main domestic animals in the Qinghai Tibet Plateau was investigated, and the genetic samples and intestinal microbial samples of the main epidemic diseases of the main domestic animals were collected. We collected Camel dung at Mohe camel farm, Ulan County, Haixi Mongolian and Tibetan Autonomous Prefecture, Qinghai Province. This data set is the 16S rRNA sequencing data of camel's intestinal microorganism in Qinghai Province. By extracting the total DNA of camel faeces, we designed primers to amplify the v3-v4 region gene fragment of bacteria, and then sequenced the corresponding data with high-throughput of the second generation. 44 samples were sequenced. This data set can be used to mine genetic resources of disease resistant individuals and find corresponding candidate genes.
DUAN Ziyuan
The content of this data set is the measurements of body weight and body size (body height, body length, chest circumference, tube circumference) of 11 representative yak populations in Qinghai pastoral area at 2018. All the metadata comes from the work of body weight monitoring of yaks in Qinghai pastoral area at 2018, by the Northwest Institute of Plateau Biology, Chinese Academy of Sciences and Qinghai Academy of Animal Husbandry and Veterinary Sciences. The data set is named by “Monitoring Data Set of Body Weights of Traditional Grazing Yaks in Qinghai Pastoral Area (2018)”, consisting of 11 worksheets. The names and contents of worksheets are as follows: 1. Haiyan-Halejing (167 yaks in halejing Mongolian Town, Haiyan County, Haibei Tibetan Autonomous Prefecture); 2. Qilian-Mole (69 yaks in Mole Town, Qilian County, Haibei Tibetan Autonomous Prefecture); 3. Qilian-Yeniugou (42 yaks in Yeniugou Town, Qilian County, Haibei Tibetan Autonomous Prefecture); 4. Qilian-Yanglong (104 yaks in Yanglong Town, Qilian County, Haibei Tibetan Autonomous Prefecture); 5. Qilian-Ebao (28 yaks in Ebao Town, Qilian County, Haibei Tibetan Autonomous Prefecture); 6. Tianjun-Xinyuan (38 yaks in Xinyuan Town, Tianjun County, Haixi Mongolian and Tibetan Autonomous Prefecture); 7. Tianjun-Longmen (100 yaks in Longmen Town, Tianjun County, Haixi Mongolian and Tibetan Autonomous Prefecture); 8. Gande-Ganlong (36 yaks in Ganglong Town, Gande County, Guoluo Tibetan Autonomous Prefecture); 9. Guinan-Taxiu (70 yaks in Taxiu Town, Guinan County, Hainan Tibetan Autonomous Prefecture); 10. Henan-Kesheng (73 yaks in Kesheng Town, Henan Mongolian Autonomous Country, Huangnan Tibetan Autonomous Prefecture); 11. Ledu-Dala (50 yaks in Dala Town, Ledu District, Haidong City). This data set comprehensively evaluates the growth performance of yaks grazing in alpine meadow under the current ecological environment through the measurement of weight and body size data in the representative areas of Qinghai pastoral area. The data set can be compared with the growth characteristics of representative populations of Qinghai yaks measured in 1981 and 2008 recorded in 1983 and 2013, and the degradation index of growth performance of yaks grazing in Qinghai pastoral area can be obtained, which is helpful to assess the impact of ecological environment changes on the growth and production performance of grazing livestock.
JIA Gongxue YANG Qien Tianwei XU
To investigate the paternal genetic structure of Tibetans from Lhasa, 1029 male samples were collected from Lhasa, Tibet. Firstly, SNP genotyping was performed to allocate samples into haplogroups. To further evaluate the genetic diversity of the major Y-chromosomal haplogroup in Tibetan populations from Lhasa, eight commonly used Y-chromosomal STR (short tandem repeat) loci (DYS19, DYS388, DYS389I, DYS389II, DYS390, DYS391, DYS392, and DYS393) were genotyped using fluorescence-labeled primers with an ABI 3130XL Genetic Analyzer (Applied Biosystems, USA). The results indicated that haplogroup D-M174 displayed highest frequency in Lhasa Tibetans (56.56%, the majority of its sublineages were D3*-P99), followed by haplogroups O-M175 (30.71%, with most of the samples belonging to O3a3c1-M117). Another relatively rare lineages in Lhasa Tibetans were N-M231 (5.15%, especially its sublineage N1*-LLY22G), C-M130 (2.62%), R-M207 (2.53%), Q (1.55%), J (0.68%), K-M, and T. Further analysis indicated that the Lhasa Tibetans’ Y chromosome haplogroups have ages within different periods, including >30 kya, LGM, post-LGM, Holocene, indicating occupation of modern humans in different periods.
KONG Qingpeng QI Xuebin
From April to June 2019, we used both live traps and camera traps to collect mammal diversity and distributions along the elevational gradients at the Yarlung Zangbo Grand Canyon National Nature Reserve. We set 64 trap lines for small mammals inventory, with a total of 11456 live trap nights. We collected 1061 individuals and 2394 tissue samples of small mammals during the field sampling. We also retrived images of 60 camera traps placed between October 2018 and April 2019. We obtained 4638 pictures of wild animals and 654 captures of anthopogenic activities. The camera traps were reset in the same locations after renew batteries and memory cards. Small mammal data consist of richness, abundance, traits, environmental gradients etc, and could be used to model relationship between environmental gradients and traits concatenated by richness matrix. Camera trap data could inventory endangered species in the region, and provide information to identify biodiversity hotspots and conservation priorities.
LI Xueyou
This data set is the transcriptome data of Tibetan pigs, which are the control group and the experimental group respectively. There are three individuals in the control group without any treatment. The experimental group is also three individuals. They are attacked with FMDV at the concentration of ID50. All samples are the transcriptome sequence results of the spleen samples of Tibetan pigs. Both the experiment and sample collection were carried out in Lanzhou. The numbers are z1-z6, and each data is divided into R1 and R2, indicating the results of double headed sequencing. Z1-Z3 is the individual results of the control group, and z4-z6 is the individual results of the experimental group. By comparing and analyzing the data of the control group and the experimental group, we can find out the response of the immune system in vivo when FMDV attacks the body of Tibetan pigs, find the immune genes and immune pathways that are activated when FMDV is attacked, find the related genes and pathways for the ability of resistance of Tibetan pigs to FMDV, and increase the immunity to FMDV in the future breeding process of domestic pigs Epidemic ability provides theoretical basis.
DUAN Ziyuan
In order to describe the distribution pattern of the genetic diversity of the main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas, and to clarify the related genetic background. In 2019, we selected two breeds of pigs in Yunnan Province as low altitude reference, collected RNA tissue samples from the brain of two breeds of pigs, extracted total RNA, built a database and sequenced the transcriptome. Sequencing produced a batch of 322g transcriptome sequencing raw data. To provide basic data for the study of the adaptation of domestic animals to the extreme environment of the Qinghai Tibet Plateau, to explore the historical events of domestication, migration and expansion of the main domesticated animals in the region, and to further explore the adaptation mechanism of domesticated animals to the poor environment such as hypoxia, high cold and dry.
PENG Minsheng
In order to describe the distribution pattern of the genetic diversity of the main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas, clarify the related genetic background, and establish the corresponding genetic resource pool. In 2019, a total of 200 samples of blood, tissue and feces of local main domesticated animals were collected in Hotan, Xinjiang Uygur Autonomous Region. This data set contains the basic sample information such as species, varieties, detailed sampling places, sample types, collection time, collectors and storage methods of sheep, Muscovy duck, pigeon, domestic chicken, domestic duck, domestic goose, domestic donkey, goat, cattle, domestic dog and other species in Hotan area of Xinjiang, which is stored in Excel form. This data set also contains the individual appearance photos of the sample, which are stored in JPG format.
Feng Xu
According to the genomic data obtained, most of the candidate genes are related to physiological development. In order to study the specific regulatory mechanism of the candidate genes, corresponding functional verification tests are carried out. Therefore, we obtained the corresponding transgenic mice and sequenced the corresponding tissues of homozygous and wild-type samples (22 tissue samples in total, including brain, bone marrow and muscle tissues). Then through the analysis of this batch of transcriptome data, we can improve the functional verification of candidate genes, in order to provide powerful data for understanding the adaptive genetic mechanism of species in different regions and physiological regulation in the process of growth and development.
LI Yan
In order to describe the distribution pattern of the genetic diversity of the main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas, clarify the related genetic background, and establish the corresponding genetic resource pool. In 2019, 406 samples of blood, tissue and feces of local main domesticated animals were collected in Luqu County, Gannan Tibetan Autonomous Prefecture, Gansu Province. This data set contains the species, varieties, detailed sampling places, sample types, collection time, collectors, storage methods and other basic sample information of sheep, pigs, yaks, chickens, cats, goats and other species in Luqu County, Qinghai Province, which are stored in Excel form. This data set also contains the individual appearance photos of the sample, which are stored in JPG format.
TIAN Fei
In order to describe the distribution pattern of the genetic diversity of the main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas, and to clarify the related genetic background. In 2019, we extracted total DNA from 21 local chicken tissue samples collected in Pakistan and Thailand, built a database and re sequenced the genome. Sequencing produced a batch of 140g genome re sequencing raw data. To provide basic data for the study of the adaptation of domestic animals to the extreme environment of the Qinghai Tibet Plateau, to explore the historical events of domestication, migration and expansion of the main domesticated animals in the region, and to further explore the adaptation mechanism of domesticated animals to the poor environment such as hypoxia, high cold and dry.
PENG Minsheng
In order to study the population evolution history and local adaptive genetic mechanism of the main domesticated equine animals in the Qinghai Tibet Plateau and its surrounding areas, and to establish the corresponding germplasm genetic resource bank. We sequenced the whole genome of 100 horse species collected in Qinghai Province, Tibet Autonomous Region and Xinjiang Autonomous Region, including Tibetan horses, Tibetan donkeys, Pingyuan donkeys and local breeds of Jiama plain. A lot of genomic data were generated by sequencing, which provided data for tracing the historical events of domestication, migration and expansion of the main domesticated equine animals in this area, and further exploring the adaptation mechanism of equine animals to the poor environment such as hypoxia, high cold and dry.
LI Yan
1) Data content: species list of amphibious and reptile in Tibet, including class, order, family, genus, species; 2) Data source and processing method: Based on the field survey of amphibians and reptiles in Tibet from 2010 to 2019, and recording the species composition and distribution range of amphibians and reptiles in this area; 3) Data quality description: the investigation, collection and identification of samples are all conducted by professionals, and the collection of samples, longitude, latitude and altitude information are checked to ensure the quality of distribution data; 4) Data application results and prospects: We selected amphibians and reptiles as model species for study. we obtained data on population size and distribution range, and provide scientific basis for assessing biodiversity pattern and formulating conservation strategies.
CHE Jing
