In order to describe the distribution pattern of genetic diversity of the main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas, clarify their genetic background, and establish the corresponding genetic resource database, the undergraduate team collected local animals in Dali, Yunnan, Shangri La, Mangkang, Luqu, Chayu, Changdu, Hetian and Yili regions from 2018 to 2019 The blood or tissue samples of the animals were collected, and the corresponding individual photos were taken at the same time. Each folder contains a set of photos of local domestic animals and a sample information sheet. Photos are stored in JPG format. The information table records the basic sample information such as species, species, detailed sampling place, sample type, collection time, collector and storage method, and stores them in the form of Excel.
YIN Tingting, PENG Minsheng
In order to collect the characteristic Domestic Animal Germplasm Resources in Qinghai Tibet Plateau and explore the molecular markers that affect the quality of the germplasm resources, the scientific research team of task five sub project 2019qzk05010704 extensively collected samples of Qinghai Tibetan sheep and Qinghai fine wool sheep in Haibei and Haixi of Qinghai Province from 2019 to 2020, and established the first and second core groups in Ledu agricultural experimental station and Sanjiaocheng sheep breeding farm of Qinghai Province. This data set contains a lambing information table, which records the lambing records of 2074 sheep. The information table includes basic sample information such as gender, lambing time, birth weight, etc., which is saved in Excel form. Individual photos were saved in JPG format and submitted to the "photo video of the second Qinghai Tibet scientific expedition (2019qzk0501) (2020)" dataset. This data set can be used in combination with physical samples to screen individual sheep with superior heterotopic sites for marker assisted selection, propagation and generation selection, and to cultivate families with special germplasm resources.
ZHAO Kai
In order to investigate the species, dispersal location and ecological impact of alien fish on the local indigenous fish in the Qinghai Tibet Plateau, the scientific research team of task 5 sub project 2019qzk05010304 investigated the lower reaches of Lhasa River and Yarlung Zangbo River from 2019 to 2020. This dataset contains a sample information table, which includes fish species, sample point information, sampling time, collector and other basic sample information, and is saved in the form of Excel. A metadata description document, saved in the form of Excel table. 160 photos were saved in JPG format and submitted to the "photo video of the second Qinghai Tibet scientific expedition (2019qzk0501) (2020)" dataset.
XIONG Wen
The birds along elevation gradients in Gangrigabu Mountains were investigated by point count method. With a 400-meter elevational gradient, elevation zones were set up in the survey area. Five elevation zones were built in the north slope from TongMai Town to Galong Temple in Bome County, and 8 elevation zones were built in the south slope from Jiefang Bridge to Galongla in Medog County. So that we can make clear about the pattern and maintenance mechanism of bird diversity along elevation gradients in this region. The data of bird diversity and distribution will be used to further explore the key scientific issues such as the impact of climate change on bird diversity and adaptation strategies, and the response and protection strategies of bird species diversity under the global climate change.
YANG Xiaojun
In November 2020, we made a collection in Qinghai Tibet Plateau were collected by net and electric capture methods, and the sampling area included the main water systems in Qinghai Province. A total of 30 sampling points were collected, and 685 fish specimens were collected in 12 points, including Schizothorax of loach.This work is a part of the project of “Building Methods for Detection of Aquatic Organisms in the Lake System of the Qinghai-Tibet Plateau”, using traditional fish survey data to generate a list of species in the lake system, which will then be used to combine multiple lakes in the plateau. High-throughput molecular data acquired from the system's environmental water samples and tested for visual parameters (lake size, isolation, geographic location, and spectral characteristics) that can be used to predict aquatic biodiversity.
LIU Shuwei
From October to November 2020, we used both live traps and camera traps to collect mammal diversity and distributions along the elevational gradients at the Yarlung Zangbo Grand Canyon National Nature Reserve. We set trap lines for small mammals inventory, with a total of 8000 live trap nights. We collected 526 individuals and1052 tissue samples of small mammals during the field sampling. We also retrived images of 130 camera traps placed between May 2020 and October 2020. We obtained 4218 pictures of wild animals,25 species of large and medium mammals were recorded.. The camera traps were reset in the same locations after renew batteries and memory cards. Small mammal data consist of richness, abundance, traits, environmental gradients etc, and could be used to model relationship between environmental gradients and traits concatenated by richness matrix. Camera trap data could inventory endangered species in the region, and provide information to identify biodiversity hotspots and conservation priorities.
LI Xueyou
The study of fossils in Bangor and Lunpola is of great significance, and the date of fossils is indispensable. There are volcanic tuffs in this area. Zircon can be used for U-Pb age analysis to determine the age of strata and fossils. This data shows the zircon U-Pb age analysis results of tuff samples from bango and Lunpola fossil sites in a graphical way. The figure shows the shape of a large number of zircons, and indicates the age analysis results on different zircon samples. The data show the large sample size used in related research, and the analysis results are also clear. The image display of this data is intuitive and clear, and the results are reliable, which is of great significance to the study of the Qinghai Tibet Plateau.
SUN Boyang
1) Data content: species list and distribution data of Phrynocephalus and Eremais in Tarim Basin, including class, order, family, genus, species, and detailed distribution information including country, province, city and county; 2) Data source and processing method: Based on the field survey of amphibians and reptiles in Tarim Basin from 2008 to 2020, and recording the species composition and distribution range of Phrynocephalus and Eremias in this area; 3) Data quality description: the investigation, collection and identification of samples are all conducted by professionals, and the collection of samples information are checked to ensure the quality of distribution data; 4) Data application results and prospects: Through comprehensive analysis of the dataset, the list of species diversity and distribution can provide important data for biodiversity cataloguing in arid central Asia, and provide scientific basis for assessing biodiversity pattern and formulating conservation strategies.
GUO Xianguang
From April 2020 to August 2020, sub project 3 collected 51 ear tissue samples of Qinghai fine wool sheep distributed in Haiyan County, Haixi Mongolian and Tibetan Autonomous Prefecture, Qinghai Province, 50 blood samples of Oula sheep in Tongde County, Hainan Tibetan Autonomous Prefecture, 50 blood samples of yak in Tongde County, Hainan Tibetan Autonomous Prefecture, 60 blood samples of Haidong donkey in Datong Hui and Tu Autonomous County, Xining City, and tissue samples A total of 211 copies. At the same time, the information of body length, body height, weight, age and gender, as well as the data of economic traits such as litter size, wool fineness and wool length were recorded. The individual photos were taken, and the information of feeding mode and epidemic situation were obtained through questionnaire survey.
TIAN Fei
In order to study the population evolution history and local adaptive genetic mechanism of main domesticated equine animals in Qinghai Tibet Plateau and its surrounding areas, and to establish the corresponding germplasm genetic resource bank. We have sequenced 236 horse samples collected in Qinghai Province, Tibet Autonomous Region and Xinjiang Autonomous Region by the end of 2018, including Tibetan horse, Tibetan donkey, plain domestic donkey and Jiama plain local breed. Seventy five samples (including 73 donkey samples and two horse samples) were sequenced for mitochondrial genome and D-loop sequencing. A number of genomic data were generated by sequencing, which provided data for tracing the domestication, migration, expansion and other historical events of horse domesticated animals in this area, and further exploring the adaptation mechanism of equine animals to the harsh environment such as hypoxia, high temperature and dryness.
LI Yan
1) Data content: including the Potential distribution map of amphipods in the Tibetan Plateau Lake. 2) The occurrence data are based on species list and distribution dataset from the Tibetan Plateau. We made potential distributions of amphipods across its range under present, Last Glacial Maximum (LGM) and mid-Holocene. 3) All the collection information are checked carefully. 4) This project, is to provide basic data for the protection of water resources and biodiversity of lakes in the Tibetan Plateau.
HOU Zhonge
In order to describe the distribution pattern of genetic diversity of main domesticated animals in Qinghai Tibet Plateau and its surrounding areas, clarify their genetic background and establish corresponding genetic resource bank. In December 2019, a total of 1208 blood or tissue samples were collected from 254 goats of Chongqing Dazu black goat, Lubei white goat, Hainan black goat, Xuzhou SuBai goat and Yimeng black goat in Chongqing, Shandong, Jiangsu and Hainan, including 9 fresh tissue RNA samples such as heart, liver, spleen, lung and kidney. This data set contains the sample species, species, detailed sampling place, sample type, collection time, collector, storage method and other basic sample information, which is stored in the form of Excel. The data set also contains the appearance photos of individual species, which are stored in JPG format.
PENG Minsheng
Data content: including the Tibetan Plateau Lake collections, the genetic diversity map of amphipods. In this project, 620 streams and lakes in the Tibetan Plateau and its surrounding areas were collected and studied for genetic diversity, in order to provide basic data for the protection of water resources and biodiversity of lakes in the Qinghai Tibet Plateau. Data source and processing method: this data set is the first-hand data, which is generated independently. The sample collection points in this data collection were obtained from four collection surveys conducted by the project team in the Tibetan Plateau from 2017 to 2020. Molecular data is to extract COI sequence from the collected samples as molecular evidence to carry out genetic diversity analysis; the main instrument is PCR, model is mastercycle x50s, manufacturer is Eppendorf. Data quality description: the data set basically covers the Tibetan Plateau and adds samples from the surrounding areas of the Qinghai Tibet Plateau. Data application achievements and prospects: provide basic data for biodiversity protection.
HOU Zhonge HOU Zhonge
In order to describe the distribution pattern of genetic diversity of main domesticated animals in Qinghai Tibet Plateau and its surrounding areas, clarify their genetic background and establish corresponding genetic resource bank. In 2020, a total of 707 blood, tissue and fecal samples from 209 domesticated animals were collected in Ili area of Xinjiang Uygur Autonomous Region, including RNA samples of heart, liver, spleen, lung and kidney of 12 horses. This data set contains the species, varieties, detailed sampling places, sample types, collection time, collectors, storage methods and other basic sample information of sheep, horses, cattle, dogs, chickens, geese, goats and other species in Yili area of Xinjiang, and is stored in Excel form. This data set also contains the appearance photos of individuals sampled and stored in JPG format.
XU Feng
In order to describe the distribution pattern of genetic diversity of the main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas (Pan third polar region), and clarify its genetic background. In 2020, we selected 31 RNA tissue samples from kidney, spleen and jejunum of local commercial chicken and native chicken in Kenya. After extracting total RNA, we established a library and performed transcriptome sequencing. Sequencing produced a batch of 180g transcriptome sequencing raw data. In order to explore the domestication, migration, expansion and other historical events of domestic chicken in Pan third pole area, and further explore the adaptation mechanism of domesticated animals to dry and other harsh environment. This data set contains the excel table of basic sample information such as species, breed, sex and phenotype of 31 domestic chicken individuals, and the original data and MD5 value of three tissue transcriptome sequencing of 31 domestic chicken individuals.
PENG Minsheng
In order to describe the distribution pattern of the genetic diversity of the main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas, clarify the related genetic background, and establish the corresponding genetic resource pool. In 2019, 187 samples of blood, tissue and feces of local main domesticated animals were collected in Chayu County, Linzhi Prefecture, Tibet Autonomous Region. This data set contains the basic sample information such as species, varieties, detailed sampling sites, sample types, collection time, collectors and storage methods of domestic chickens, pigs, cattle, horses, dogs and buffaloes in Chayu County, Linzhi, Tibet, and is stored in Excel form. This data set also contains the individual appearance photos of the sample, which are stored in JPG format.
PENG Minsheng, YIN Tingting
In order to describe the disease situation of the main domesticated animals in and around the Qinghai Tibet Plateau, the epidemic situation of the main domestic animals in the Qinghai Tibet Plateau was investigated, and the genetic samples and intestinal microbial samples of the main epidemic diseases of the main domestic animals were collected. We collected Camel dung at Mohe camel farm, Ulan County, Haixi Mongolian and Tibetan Autonomous Prefecture, Qinghai Province. This data set is the 16S rRNA sequencing data of camel's intestinal microorganism in Qinghai Province. By extracting the total DNA of camel faeces, we designed primers to amplify the v3-v4 region gene fragment of bacteria, and then sequenced the corresponding data with high-throughput of the second generation. 44 samples were sequenced. This data set can be used to mine genetic resources of disease resistant individuals and find corresponding candidate genes.
DUAN Ziyuan
The content of this data set is the measurements of body weight and body size (body height, body length, chest circumference, tube circumference) of 11 representative yak populations in Qinghai pastoral area at 2018. All the metadata comes from the work of body weight monitoring of yaks in Qinghai pastoral area at 2018, by the Northwest Institute of Plateau Biology, Chinese Academy of Sciences and Qinghai Academy of Animal Husbandry and Veterinary Sciences. The data set is named by “Monitoring Data Set of Body Weights of Traditional Grazing Yaks in Qinghai Pastoral Area (2018)”, consisting of 11 worksheets. The names and contents of worksheets are as follows: 1. Haiyan-Halejing (167 yaks in halejing Mongolian Town, Haiyan County, Haibei Tibetan Autonomous Prefecture); 2. Qilian-Mole (69 yaks in Mole Town, Qilian County, Haibei Tibetan Autonomous Prefecture); 3. Qilian-Yeniugou (42 yaks in Yeniugou Town, Qilian County, Haibei Tibetan Autonomous Prefecture); 4. Qilian-Yanglong (104 yaks in Yanglong Town, Qilian County, Haibei Tibetan Autonomous Prefecture); 5. Qilian-Ebao (28 yaks in Ebao Town, Qilian County, Haibei Tibetan Autonomous Prefecture); 6. Tianjun-Xinyuan (38 yaks in Xinyuan Town, Tianjun County, Haixi Mongolian and Tibetan Autonomous Prefecture); 7. Tianjun-Longmen (100 yaks in Longmen Town, Tianjun County, Haixi Mongolian and Tibetan Autonomous Prefecture); 8. Gande-Ganlong (36 yaks in Ganglong Town, Gande County, Guoluo Tibetan Autonomous Prefecture); 9. Guinan-Taxiu (70 yaks in Taxiu Town, Guinan County, Hainan Tibetan Autonomous Prefecture); 10. Henan-Kesheng (73 yaks in Kesheng Town, Henan Mongolian Autonomous Country, Huangnan Tibetan Autonomous Prefecture); 11. Ledu-Dala (50 yaks in Dala Town, Ledu District, Haidong City). This data set comprehensively evaluates the growth performance of yaks grazing in alpine meadow under the current ecological environment through the measurement of weight and body size data in the representative areas of Qinghai pastoral area. The data set can be compared with the growth characteristics of representative populations of Qinghai yaks measured in 1981 and 2008 recorded in 1983 and 2013, and the degradation index of growth performance of yaks grazing in Qinghai pastoral area can be obtained, which is helpful to assess the impact of ecological environment changes on the growth and production performance of grazing livestock.
JIA Gongxue, YANG Qien, Tianwei XU
To investigate the paternal genetic structure of Tibetans from Lhasa, 1029 male samples were collected from Lhasa, Tibet. Firstly, SNP genotyping was performed to allocate samples into haplogroups. To further evaluate the genetic diversity of the major Y-chromosomal haplogroup in Tibetan populations from Lhasa, eight commonly used Y-chromosomal STR (short tandem repeat) loci (DYS19, DYS388, DYS389I, DYS389II, DYS390, DYS391, DYS392, and DYS393) were genotyped using fluorescence-labeled primers with an ABI 3130XL Genetic Analyzer (Applied Biosystems, USA). The results indicated that haplogroup D-M174 displayed highest frequency in Lhasa Tibetans (56.56%, the majority of its sublineages were D3*-P99), followed by haplogroups O-M175 (30.71%, with most of the samples belonging to O3a3c1-M117). Another relatively rare lineages in Lhasa Tibetans were N-M231 (5.15%, especially its sublineage N1*-LLY22G), C-M130 (2.62%), R-M207 (2.53%), Q (1.55%), J (0.68%), K-M, and T. Further analysis indicated that the Lhasa Tibetans’ Y chromosome haplogroups have ages within different periods, including >30 kya, LGM, post-LGM, Holocene, indicating occupation of modern humans in different periods.
KONG Qingpeng, QI Xuebin
From April to June 2019, we used both live traps and camera traps to collect mammal diversity and distributions along the elevational gradients at the Yarlung Zangbo Grand Canyon National Nature Reserve. We set 64 trap lines for small mammals inventory, with a total of 11456 live trap nights. We collected 1061 individuals and 2394 tissue samples of small mammals during the field sampling. We also retrived images of 60 camera traps placed between October 2018 and April 2019. We obtained 4638 pictures of wild animals and 654 captures of anthopogenic activities. The camera traps were reset in the same locations after renew batteries and memory cards. Small mammal data consist of richness, abundance, traits, environmental gradients etc, and could be used to model relationship between environmental gradients and traits concatenated by richness matrix. Camera trap data could inventory endangered species in the region, and provide information to identify biodiversity hotspots and conservation priorities.
LI Xueyou
